Using deuterated PAH amendments to validate chemical extraction methods to predict PAH bioavailability in soils

Validating chemical methods to predict bioavailable fractions of polycyclic aromatic hydrocarbons (PAHs) by comparison with accumulation bioassays is problematic. Concentrations accumulated in soil organisms not only depend on the bioavailable fraction but also on contaminant properties. A historically contaminated soil was freshly spiked with deuterated PAHs (dPAHs). dPAHs have a similar fate to their respective undeuterated analogues, so chemical methods that give good indications of bioavailability should extract the fresh more readily available dPAHs and historic more recalcitrant PAHs in similar proportions to those in which they are accumulated in the tissues of test organisms. Cyclodextrin and butanol extractions predicted the bioavailable fraction for earthworms (Eisenia fetida) and plants (Lolium multiflorum) better than the exhaustive extraction. The PAHs accumulated by earthworms had a larger dPAH:PAH ratio than that predicted by chemical methods. The isotope ratio method described here provides an effective way of evaluating other chemical methods to predict bioavailability

The Stellar Mass Density at z~6 from Spitzer Imaging of i-drop Galaxies

We measure the ages, stellar masses, and star formation histories of z~6 galaxies, observed within 1Gyr of the Big Bang. We use imaging from HST and Spitzer from the public "Great Observatories Origins Deep Survey", coupled with ground-based near-infrared imaging, to measure the spectral energy distributions from 0.8-5microns, spanning the rest-frame UV and optical. From our sample of ~50 i-drop Lyman-break star-forming galaxies in GOODS-South with z'(AB)<27mag, we focus on ~30 with reliable photometric or spectroscopic redshifts. Half of these are confused with foreground sources at Spitzer resolution, but from the 16 with clean photometry we find that a surprisingly large fraction (40%) have evidence for substantial Balmer-breaks. This indicates the presence of old underlying stellar populations that dominate the stellar masses. For these objects, we find ages of 200-700Myr, implying formation redshifts of 7<z<14, and large stellar masses in the range 1-3x10^10M_sun. Analysis of 7 i-drops that are undetected at 3.6microns indicates that these are younger, considerably less massive systems. We calculate that line contamination should not severely affect our photometry or derived results. Using data out to 8microns, we find little evidence for substantial intrinsic dust reddening. Correcting for incompleteness in our sample, we find a lower limit on the comoving stellar mass density at z~6 to be 2.5x10^6M_sun/Mpc^3. We are able to explore the star formation histories of our selected galaxies, and we infer that the past global star formation rate may have been much higher than that observed at z~6. The associated UV flux we infer at z>7 could have played a major role in reionizing the universe.Comment: Accepted for publication in MNRAS. Minor changes in response to referee repor

Relative proportions of polycyclic aromatic hydrocarbons differ between accumulation bioassays and chemical methods to predict bioavailability

Chemical methods to predict the bioavailable fraction of organic contaminants are usually validated in the literature by comparison with established bioassays. A soil spiked with polycyclic aromatic hydrocarbons (PAHs) was aged over six months and subjected to butanol, cyclodextrin and tenax extractions as well as an exhaustive extraction to determine total PAH concentrations at several time points. Earthworm (Eisenia fetida) and rye grass root (Lolium multiflorum) accumulation bioassays were conducted in parallel. Butanol extractions gave the best relationship with earthworm accumulation (r2 ≤ 0.54, p ≤ 0.01); cyclodextrin, butanol and acetone–hexane extractions all gave good predictions of accumulation in rye grass roots (r2 ≤ 0.86, p ≤ 0.01). However, the profile of the PAHs extracted by the different chemical methods was significantly different (p < 0.01) to that accumulated in the organisms. Biota accumulated a higher proportion of the heavier 4-ringed PAHs. It is concluded that bioaccumulation is a complex process that cannot be predicted by measuring the bioavailable fraction alone. The ability of chemical methods to predict PAH accumulation in Eisenia fetida and Lolium multiflorum was hindered by the varied metabolic fate of the different PAHs within the organisms

Effects of Vitamin D Supplementation on Cognitive and Emotional Functioning in Young Adults – A Randomised Controlled Trial

Background: Epidemiological research links vitamin D status to various brain-related outcomes. However, few trials examine whether supplementation can improve such outcomes and none have examined effects on cognition. This study examined whether Vitamin D supplementation led to improvements in diverse measures of cognitive and emotional functioning, and hypothesised that supplementation would lead to improvements in these outcomes compared to placebo

SARS-CoV-2 Infection and the Risk of Suicidal and Self-Harm Thoughts and Behaviour:A Systematic Review

OBJECTIVE: The COVID-19 pandemic has had a complex impact on risks of suicide and non-fatal self-harm worldwide with some evidence of increased risk in specific populations including women, young people, and people from ethnic minority backgrounds. This review aims to systematically address whether SARS-CoV-2 infection and/or COVID-19 disease confer elevated risk directly. METHOD: As part of a larger Living Systematic Review examining self-harm and suicide during the pandemic, automated daily searches using a broad list of keywords were performed on a comprehensive set of databases with data from relevant articles published between January 1, 2020 and July 18, 2021. Eligibility criteria for our present review included studies investigating suicide and/or self-harm in people infected with SARS-CoV-2 with or without manifestations of COVID-19 disease with a comparator group who did not have infection or disease. Suicidal and self-harm thoughts and behaviour (STBs) were outcomes of interest. Studies were excluded if they reported data for people who only had potential infection/disease without a confirmed exposure, clinical/molecular diagnosis or self-report of a positive SARS-CoV-2 test result. Studies of news reports, treatment studies, and ecological studies examining rates of both SARS-CoV-2 infections and suicide/self-harm rates across a region were also excluded. RESULTS: We identified 12 studies examining STBs in nine distinct samples of people with SARS-CoV-2. These studies, which investigated STBs in the general population and in subpopulations, including healthcare workers, generally found positive associations between SARS-CoV-2 infection and/or COVID-19 disease and subsequent suicidal/self-harm thoughts and suicidal/self-harm behaviour. CONCLUSIONS: This review identified some evidence that infection with SARS-CoV-2 and/or COVID-19 disease may be associated with increased risks for suicidal and self-harm thoughts and behaviours but a causal link cannot be inferred. Further research with longer follow-up periods is required to confirm these findings and to establish whether these associations are causal

Spitzer Imaging of i'-drop Galaxies: Old Stars at z~6

We present new evidence for mature stellar populations with ages >100Myr in massive galaxies (M_stellar>10^10M_sun) seen at a time when the Universe was less than 1Gyr old. We analyse the prominent detections of two z~6 star-forming galaxies (SBM03#1 & #3) made at wavelengths corresponding to the rest-frame optical using the IRAC camera onboard the Spitzer Space Telescope. We had previously identified these galaxies in HST/ACS GOODS images of Chandra Deep Field South through the "i-drop" Lyman break technique, and subsequently confirmed spectroscopically with the Keck telescope. The new Spitzer photometry reveals significant Balmer/4000Ang discontinuities, indicative of dominant stellar populations with ages >100Myr. Fitting a range of population synthesis models (for normal initial mass functions) to the HST/Spitzer photometry yields ages of 250-650Myr and implied formation redshifts z~7.5-13.5 in presently-accepted world models. Remarkably, our sources have best-fit stellar masses of 1.3-3.8x10^10M_sun (95% confidence) assuming a Salpeter initial mass function. This indicates that at least some galaxies with stellar masses >20% of those of a present-day L* galaxy had already assembled within the first Gyr after the Big Bang. We also deduce that the past average star formation rate must be comparable to the current observed rate (SFR_UV~5-30M_sun/yr), suggesting that there may have been more vigorous episodes of star formation in such systems at higher redshifts. Although a small sample, limited primarily by Spitzer's detection efficiency, our result lends support to the hypothesis advocated in our earlier analyses of the Ultra Deep Field and GOODS HST/ACS data. The presence of established systems at z~6 suggests long-lived sources at earlier epochs (z>7) played a key role in reionizing the Universe.Comment: Accepted for publication in MNRAS (minor corrections made

Star Forming Galaxies at z~6 and Reionization

We determine the abundance of i'-band drop-outs in the HST/ACS GOODS surveys and the Hubble Ultra Deep Field (UDF). The majority of these sources are likely to be z~6 galaxies whose flux decrement arises from Lyman-alpha absorption. We have shown with Keck/DEIMOS spectroscopy that this technique does indeed select high redshift galaxies, and we discovered Lyman-alpha emission for about a third of the galaxies with z'_AB<25.6 The increased depth of UDF enables us to reach a ~10sigma limiting magnitude of z'_AB=28.5 (equivalent to 1.5M_sun/yr at z=6.1, or 0.1L*_UV for the z~3 U-drop population). The star formation rate at z~6 was approximately x6 LESS than at z~3. This declining comoving star formation rate poses an interesting challenge for models which suggest that L_UV>0.1L* star forming galaxies at z~6 reionized the universe. The short-fall in ionizing photons might be alleviated by galaxies fainter than our limit, or a radically different IMF. Alternatively, the bulk of reionization might have occurred at z>>6. We have recently discovered evidence of an early epoch of star formation in some of the i'-drops at z~6. Spitzer images with IRAC at 3.6-4.5microns show evidence of the age-sensitive Balmer/4000Ang break, dominated by stars older than 100Myr (and most probably 400Myr old). This pushes the formation epoch for these galaxies to z_form=7.5-13.5. There are at least some galaxies already assembled with stellar masses ~3x10^10M_sun (equivalent to 0.2M* today) within the first billion years. The early formation of such systems may have played a key role in reionizing the Universe at z~10.Comment: To appear in proceedings of UC Irvine May 2005 workshop on "First Light & Reionization", eds. E. Barton & A. Cooray, New Astronomy Reviews, in pres